Tilling Genes

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TILLING is to identify the mutants with trait of interest.

Strategy

  • Develop a TILLING population
  • Select/isolate the gene of interest (direct or orthologous)
  • Identify SNP
  • Screen the mutants with specific strategy

Conformation of Selected Genes

  • Blast against methylated (TGI) sequences
    • 7 selected genes
    • Blastn against TGI []
    • Results
Query id	ANTL_AY461432
Subject id	CHO_OF4928xj19r1.ab1
% identity	99.39
alignment length	654
mismatches	2
gap openings	2
q. start	1676
q. end	2327
s. start	848
s. end	195
e-value	0
bit score	1189
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