Tilling Genes

TILLING is to identify the mutants with trait of interest.

Strategy

• Develop a TILLING population
• Select/isolate the gene of interest (direct or orthologous)
• Identify SNP
• Screen the mutants with specific strategy

Conformation of Selected Genes

• Blast against methylated (TGI) sequences
  • 7 selected genes
  • Blastn against TGI []
  • Results

Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
ANTL_AY461432	CHO_OF4928xj19r1.ab1	99.39	654	2	2	1676	2327	848	195	0.0	1189