Tilling Genes

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TILLING is to identify the mutants with trait of interest.

Strategy

Develop a TILLING population
Select/isolate the gene of interest (direct or orthologous)
Identify SNP
Screen the mutants with specific strategy

Conformation of Selected Genes

Blast against methylated (TGI) sequences
- 7 selected genes

ANTL_AY461432
LEMTS1_Tomato)Linalool_Synthase_AY840091
LeMTS2
NtLs_EU935581_Lateral_suppressor
AtTRIPTYCHON
AtPPD2
AtPPD1

- Blastn against TGI [singlet and contig]
- Results with TGI singlets

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Bioinformatics