Tilling Genes

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TILLING is to identify the mutants with trait of interest.

Strategy

  • Develop a TILLING population
  • Select/isolate the gene of interest (direct or orthologous)
  • Identify SNP
  • Screen the mutants with specific strategy

Conformation of Selected Genes

  • Blast against methylated (TGI) sequences
    • 7 selected genes
    • Blastn against TGI []
    • Results withTGI singlets
Query id	ANTL_AY461432		NtLs_EU935581_Lateral_suppressor
Subject id	CHO_OF4928xj19r1.ab1	CHO_OF3339xj20r1.ab1
% identity	99.39			96.59
aln length	654			821
mismatches	2			17
gap openings	2			3
q. start	1676			271
q. end		2327			1081
s. start	848			1
s. end		195			820
e-value		0			0
bit score	1189			1265
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