MolBio Meet

2010

4NOV2010

• Raj
  • Pollen dynamics - completed - MLTR - to be digged through..
  • SSR Pipe - completed and pilot set sent for oligo synthesis
  • Casuarina genome sequecing - status - Presentation completed. Next with Pradeep, Kulkarni
  • TD-GD scoring, analysis and depiction
  • Tilling Genes confirmed
  • Transcriptomics-Tobacco: Mapping ESTs using bFast (new strategy)
  • Digged thorough Gene prediction (completed genewise and genscan)
  • Completed : 2010-11 budget, Flavour vision presentation
• Prasad
  • Performance slides
  • Finalyse the money part in CSIRO trip
  • Could finalise 25 genes of growth related with partial sequences
  • To lead stage wise expression studies of isolated genes
• Ashoke
  • Performance slides
  • Finalyse 2 selective profile this week in SEX marker
  • His part of TD-GD over
• Bhushan
  • Performance slides
  • Sent pilot set of Ortho-SSR and follow up
  • Remaining Gels of TD-GD (5 more)

Appraisal-APR2010

• Prasad

Prasad Hendre has successfully completed the committed work on gene isolation despite of practical difficulties, particularly in cloning. He was also instrumental in trouble shooting team members' problems. He was highly focused in his deliverables. Hendre is critical in discussions and assess the risk factors well in advance. Some of the minor gaps in which he has to work on are

• • Looking the problem in a wholistic way and out of the box thinking
  • Revamp his breeding expertise to appropriately fit in MolbBio experiments.
  • Informal discussion with silvi team with more fine tuned communication skills to more understand the specific issues of individual projects

The above gaps, when taken care, he would turn to be an excellent scientist for the center.

• • Action Points
    • Develop fine tuned communication skills (learn the principle of "give it to get it")
    • Develop know-how in Tree genetics and population dynamics
    • Work for a common cause.
• Bhushan

Nagabhushana was given the target of cellulose synthesis gene family isolation. He critically selected 6 major genes, of which 2 have been isolated, in addition to 4 promoters. There was a mid-term correction after serious discussion with collaborators, to withdraw CesA gene family gene isolation to avoid future redundancy. Bhushana was given other responsibilities that include wet-validation of Ortho-SSRs; and pilot experiment on GD of new clones, which he completed in time with success. Nagabhushana proved to be a good team worker in work flow and discussions. He is well aware of center's over all objectives as evident from many of this discussions and attitude. Few additional gaps, in which he has to show his potential are,

• • Active involvement and enthusiasm to escape from being trapped by comfort zone.
  • Every day micro-level planning is solicited and self - review the same.
  • Situational leadership is solicited ignoring the hierarchy.

The above gaps, when taken care, he would turn to be an excellent resource for the center.

• • Action Points
    • Activeness and get-out of comfort zone
    • Learn to switch between work entities quickly
    • Micro-level planning and self-review with respect to time line
• Ashok

Ashok has consistently shown good work flow in gene isolation and Casuarina project. He has successfully isolated 10 cell wall bio synthesis gene in E. camaldulensis in both direct and heterologous approaches. He was also reviewed the genes through expression analysis for its suitability. In addition, Ashok undertook a minor project to identify sex-specific marker in Casuarina, wherein he is presently characterizing two of the sex-specific marker identified. Ashok is highly task focused and a good team worker. Couple of areas in which he has to work are,

• • Intensive discussion within and outside the domain.
  • Needs thorough understanding of overview of the project
  • Needs more interaction in the common forum (seminars and meetings)

The above gaps, when taken care, he would turn to be an excellent resource for the center.

• • Action points
    • Interaction
    • Logical reasoning
    • Seek for theoretical know-how (reading) and

2009

15MAY2009

• Discussion on Introduction
• Nagabhusan
  • Reviewed the work
  • Discussed on siRNA feasibilities
• Prasad
  • Reviewed the work
  • Need the deliverables to be concised
• Ashoke
  • Reviewed the work
  • Discussed on Expansin localization

22MAY2009

• Discussed on Poster
  • Association mapping
  • Molecular markers as tools [ITC clones diversity; Hybrid confirmation; Model Natural Hybridization; Sex in Casuarina]
• XET gene (Ashoke) PCR amplification was trouble shooted (by Prasad)

05JUN2009

• Progress
  • Raj
    • Project GeneFlow
      • DNA Isolation from parents completed | Quantified | Working solution made
      • Plants resown for gap filling of projenies
      • Plants will be ready in 10 days time
      • Periodical thinning is suggested for DNA isolation from Monday
      • Primer screening to be started
    • Project :: Frankia
      • DNA isolated from 3 species | pathetic quantity
      • Primers are ready for PCR
  • Prasad
    • Gene walking
    • Expression studies
  • Ashoke
    • Gene Walking
      • Expansin Gene | Cloning completed | to be sent for sequencing
    • Expression studies
      • Ready Sample collection form different aged plants
  • Nagabhusana
    • Gene walking
    • Expression studies

12JUN2009

• Proof of principle - evagen - what followhup
  • It is possible to outsource the proof of principle
• RAC - by Mid JULY
• Casuarina Plan
  • Accepted to get it done from Kovai
  • Species : C. juhuniana
  • Male Female bulks are possible
• Change in timings to 3.30 pm on Friday
• Status
  • RAJ : DNA Isolation
  • PRASAD : Close to 80% of the gene enrichment protocol
  • ASHOK : Expansin Walking Issues
  • NAGA : Missing

19JUN2009

• Status
  • RAJ: Primer Screening ; Suggested to use the existing primers
  • Ashoke
    • Expansin - the primers didn't work. To design new primers (degenerate)
    • RACE - started and amplified first set of products
    • Plan for Seminar
  • Nagabhusana
    • CES A: Sent for sequencing
  • Prasad

26JUN2009

• Agenda
  • Sample collection | Stress makes any difference ?
  • AFLP Kit ordering
  • Raj
    • Primers are fine, but HS Agarose is non-sense

17AUG2009

• Common Stuff
  • DNTPs (Requirement from my side 600ul of 80ug)
  • Acrylamide plates order follow up - Nagabhushan
  • AFLP kit status - Ashoke to forward the message
  • LabNotebook, how do we deal it - sign every week
  • Equipments to Gurpreet: 1 PCR, 1 RT, 1 table top Centrifuge
  • Paper writing
  • SOL Meeting 2009 | last date for abstract 25AUG2009 - nothing this time
• Raj
  • Outcrssing rate work looks intensive
  • Prasad already had a procedure for beat beating in DNA isoaltion
• Prasad
  • Status - Gene enrichment protocol
  • Problem with trasformation - trouble shooting it
• Ashoke
  • Status
  • Problem with trasformation - trouble shooting it
• Bhushan
  • Status on SSR
  • Status on gene isolation