MolBio Meet

2010

3SEP2010

• Raj
  • Status Casuarina Genome sequencing
  • Outcrossing Needs to be compled and presendted on Monday
  • Collect RNA samples for biomass gene targets
• Prasad
  • Association DNA completed and sent:
  • Working on 50 additional genes and organising for primer sysnthesis
  • Planning on staructure analysis before the CSIRO
• Ashok
  • Validation population (targetting Biomass genes): Completed the 580 plants DNA isolation
  • Quality and Quantity check completed
  • Making samples ready for Sending
• Nagabhushana
  • SSR: Ordered 50 additional primers | Checking is in progress
  • Hybrid confirmation: Needs to re-collect the samples
  • TD-GD discussion

Appraisal-APR2010

Appraisal

4NOV2010

• Raj
  • Pollen dynamics - completed - MLTR - to be digged through..
  • SSR Pipe - completed and pilot set sent for oligo synthesis
  • Casuarina genome sequecing - status - Presentation completed. Next with Pradeep, Kulkarni
  • TD-GD scoring, analysis and depiction
  • Tilling Genes confirmed
  • Transcriptomics-Tobacco: Mapping ESTs using bFast (new strategy)
  • Digged thorough Gene prediction (completed genewise and genscan)
  • Completed : 2010-11 budget, Flavour vision presentation
• Prasad
  • Performance slides
  • Finalyse the money part in CSIRO trip
  • Could finalise 25 genes of growth related with partial sequences
  • To lead stage wise expression studies of isolated genes
• Ashoke
  • Performance slides
  • Finalyse 2 selective profile this week in SEX marker
  • His part of TD-GD over
• Bhushan
  • Performance slides
  • Sent pilot set of Ortho-SSR and follow up
  • Remaining Gels of TD-GD (5 more)

2009

15MAY2009

• Discussion on Introduction
• Nagabhusan
  • Reviewed the work
  • Discussed on siRNA feasibilities
• Prasad
  • Reviewed the work
  • Need the deliverables to be concised
• Ashoke
  • Reviewed the work
  • Discussed on Expansin localization

22MAY2009

• Discussed on Poster
  • Association mapping
  • Molecular markers as tools [ITC clones diversity; Hybrid confirmation; Model Natural Hybridization; Sex in Casuarina]
• XET gene (Ashoke) PCR amplification was trouble shooted (by Prasad)

05JUN2009

• Progress
  • Raj
    • Project GeneFlow
      • DNA Isolation from parents completed | Quantified | Working solution made
      • Plants resown for gap filling of projenies
      • Plants will be ready in 10 days time
      • Periodical thinning is suggested for DNA isolation from Monday
      • Primer screening to be started
    • Project :: Frankia
      • DNA isolated from 3 species | pathetic quantity
      • Primers are ready for PCR
  • Prasad
    • Gene walking
    • Expression studies
  • Ashoke
    • Gene Walking
      • Expansin Gene | Cloning completed | to be sent for sequencing
    • Expression studies
      • Ready Sample collection form different aged plants
  • Nagabhusana
    • Gene walking
    • Expression studies

12JUN2009

• Proof of principle - evagen - what followhup
  • It is possible to outsource the proof of principle
• RAC - by Mid JULY
• Casuarina Plan
  • Accepted to get it done from Kovai
  • Species : C. juhuniana
  • Male Female bulks are possible
• Change in timings to 3.30 pm on Friday
• Status
  • RAJ : DNA Isolation
  • PRASAD : Close to 80% of the gene enrichment protocol
  • ASHOK : Expansin Walking Issues
  • NAGA : Missing

19JUN2009

• Status
  • RAJ: Primer Screening ; Suggested to use the existing primers
  • Ashoke
    • Expansin - the primers didn't work. To design new primers (degenerate)
    • RACE - started and amplified first set of products
    • Plan for Seminar
  • Nagabhusana
    • CES A: Sent for sequencing
  • Prasad

26JUN2009

• Agenda
  • Sample collection | Stress makes any difference ?
  • AFLP Kit ordering
  • Raj
    • Primers are fine, but HS Agarose is non-sense

17AUG2009

• Common Stuff
  • DNTPs (Requirement from my side 600ul of 80ug)
  • Acrylamide plates order follow up - Nagabhushan
  • AFLP kit status - Ashoke to forward the message
  • LabNotebook, how do we deal it - sign every week
  • Equipments to Gurpreet: 1 PCR, 1 RT, 1 table top Centrifuge
  • Paper writing
  • SOL Meeting 2009 | last date for abstract 25AUG2009 - nothing this time
• Raj
  • Outcrssing rate work looks intensive
  • Prasad already had a procedure for beat beating in DNA isoaltion
• Prasad
  • Status - Gene enrichment protocol
  • Problem with trasformation - trouble shooting it
• Ashoke
  • Status
  • Problem with trasformation - trouble shooting it
• Bhushan
  • Status on SSR
  • Status on gene isolation