Tilling Genes: Difference between revisions
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* Blast against methylated (TGI) sequences | * Blast against methylated (TGI) sequences | ||
** 7 selected genes | ** 7 selected genes | ||
<pre> | |||
<pre> | <pre> | ||
ANTL_AY461432 | ANTL_AY461432 | ||
| Line 20: | Line 18: | ||
AtPPD2 | AtPPD2 | ||
AtPPD1 | AtPPD1 | ||
</pre> | </pre> | ||
** Blastn against TGI [singlet and contig] | |||
** Results with TGI singlets | |||
Revision as of 09:57, 6 January 2010
TILLING is to identify the mutants with trait of interest.
Strategy
- Develop a TILLING population
- Select/isolate the gene of interest (direct or orthologous)
- Identify SNP
- Screen the mutants with specific strategy
Conformation of Selected Genes
- Blast against methylated (TGI) sequences
- 7 selected genes
<pre> ANTL_AY461432 LEMTS1_Tomato)Linalool_Synthase_AY840091 LeMTS2 NtLs_EU935581_Lateral_suppressor AtTRIPTYCHON AtPPD2 AtPPD1
- Blastn against TGI [singlet and contig]
- Results with TGI singlets