Tilling Genes: Difference between revisions
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| Line 9: | Line 9: | ||
* Blast against methylated (TGI) sequences | * Blast against methylated (TGI) sequences | ||
** 7 selected genes | ** 7 selected genes | ||
** | |||
** Blastn against TGI [singlet and contig] | ** Blastn against TGI [singlet and contig] | ||
** Results withTGI singlets | ** Results withTGI singlets | ||
<pre> | <pre> | ||
ANTL_AY461432 | |||
LEMTS1_Tomato)Linalool_Synthase_AY840091 | |||
LeMTS2 | |||
NtLs_EU935581_Lateral_suppressor | |||
AtTRIPTYCHON | |||
AtPPD2 | |||
AtPPD1 | |||
</pre> | </pre> | ||
Revision as of 09:51, 6 January 2010
TILLING is to identify the mutants with trait of interest.
Strategy
- Develop a TILLING population
- Select/isolate the gene of interest (direct or orthologous)
- Identify SNP
- Screen the mutants with specific strategy
Conformation of Selected Genes
- Blast against methylated (TGI) sequences
- 7 selected genes
- Blastn against TGI [singlet and contig]
- Results withTGI singlets
ANTL_AY461432 LEMTS1_Tomato)Linalool_Synthase_AY840091 LeMTS2 NtLs_EU935581_Lateral_suppressor AtTRIPTYCHON AtPPD2 AtPPD1