Tilling Genes: Difference between revisions
Jump to navigation
Jump to search
| Line 9: | Line 9: | ||
* Blast against methylated (TGI) sequences | * Blast against methylated (TGI) sequences | ||
** 7 selected genes | ** 7 selected genes | ||
** Blastn against TGI [] | ** Blastn against TGI [singlet and contig] | ||
** Results withTGI singlets | ** Results withTGI singlets | ||
<pre> | <pre> | ||
Revision as of 18:30, 5 January 2010
TILLING is to identify the mutants with trait of interest.
Strategy
- Develop a TILLING population
- Select/isolate the gene of interest (direct or orthologous)
- Identify SNP
- Screen the mutants with specific strategy
Conformation of Selected Genes
- Blast against methylated (TGI) sequences
- 7 selected genes
- Blastn against TGI [singlet and contig]
- Results withTGI singlets
Query id ANTL_AY461432 NtLs_EU935581_Lateral_suppressor Subject id CHO_OF4928xj19r1.ab1 CHO_OF3339xj20r1.ab1 % identity 99.39 96.59 aln length 654 821 mismatches 2 17 gap openings 2 3 q. start 1676 271 q. end 2327 1081 s. start 848 1 s. end 195 820 e-value 0 0 bit score 1189 1265