Tilling Genes: Difference between revisions
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| Line 12: | Line 12: | ||
** Results | ** Results | ||
<pre> | <pre> | ||
Query id | Query id ANTL_AY461432 | ||
Subject id CHO_OF4928xj19r1.ab1 | |||
% identity 99.39 | |||
alignment length 654 | |||
mismatches 2 | |||
gap openings 2 | |||
q. start 1676 | |||
q. end 2327 | |||
s. start 848 | |||
s. end 195 | |||
e-value 0 | |||
bit score 1189 | |||
</pre> | </pre> | ||
Revision as of 18:19, 5 January 2010
TILLING is to identify the mutants with trait of interest.
Strategy
- Develop a TILLING population
- Select/isolate the gene of interest (direct or orthologous)
- Identify SNP
- Screen the mutants with specific strategy
Conformation of Selected Genes
- Blast against methylated (TGI) sequences
- 7 selected genes
- Blastn against TGI []
- Results
Query id ANTL_AY461432 Subject id CHO_OF4928xj19r1.ab1 % identity 99.39 alignment length 654 mismatches 2 gap openings 2 q. start 1676 q. end 2327 s. start 848 s. end 195 e-value 0 bit score 1189