Tilling Genes: Difference between revisions
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| Line 9: | Line 9: | ||
* Blast against methylated (TGI) sequences | * Blast against methylated (TGI) sequences | ||
** 7 selected genes | ** 7 selected genes | ||
<pre> | <pre> | ||
ANTL_AY461432 | ANTL_AY461432 | ||
Revision as of 10:28, 6 January 2010
TILLING is to identify the mutants with trait of interest.
Strategy
- Develop a TILLING population
- Select/isolate the gene of interest (direct or orthologous)
- Identify SNP
- Screen the mutants with specific strategy
Conformation of Selected Genes
- Blast against methylated (TGI) sequences
- 7 selected genes
ANTL_AY461432 LEMTS1_Tomato)Linalool_Synthase_AY840091 LeMTS2 NtLs_EU935581_Lateral_suppressor AtTRIPTYCHON AtPPD2 AtPPD1
- Blastn against TGI [singlet and contig]
- Results with TGI singlets