Tilling Genes: Difference between revisions
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| Line 10: | Line 10: | ||
** 7 selected genes | ** 7 selected genes | ||
** Blastn against TGI [] | ** Blastn against TGI [] | ||
** Results | ** Results withTGI singlets | ||
<pre> | <pre> | ||
Query id ANTL_AY461432 | Query id ANTL_AY461432 NtLs_EU935581_Lateral_suppressor | ||
Subject id CHO_OF4928xj19r1.ab1 | Subject id CHO_OF4928xj19r1.ab1 CHO_OF3339xj20r1.ab1 | ||
% identity 99.39 | % identity 99.39 96.59 | ||
alignment length 654 | alignment length 654 821 | ||
mismatches 2 | mismatches 2 17 | ||
gap openings 2 | gap openings 2 3 | ||
q. start 1676 | q. start 1676 271 | ||
q. end 2327 | q. end 2327 1081 | ||
s. start 848 | s. start 848 1 | ||
s. end 195 | s. end 195 820 | ||
e-value 0 | e-value 0 0 | ||
bit score 1189 | bit score 1189 1265 | ||
Revision as of 18:26, 5 January 2010
TILLING is to identify the mutants with trait of interest.
Strategy
- Develop a TILLING population
- Select/isolate the gene of interest (direct or orthologous)
- Identify SNP
- Screen the mutants with specific strategy
Conformation of Selected Genes
- Blast against methylated (TGI) sequences
- 7 selected genes
- Blastn against TGI []
- Results withTGI singlets
Query id ANTL_AY461432 NtLs_EU935581_Lateral_suppressor Subject id CHO_OF4928xj19r1.ab1 CHO_OF3339xj20r1.ab1 % identity 99.39 96.59 alignment length 654 821 mismatches 2 17 gap openings 2 3 q. start 1676 271 q. end 2327 1081 s. start 848 1 s. end 195 820 e-value 0 0 bit score 1189 1265