MolBio Meet: Difference between revisions

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=15MAY2009=
[[Category:Meetings]]
 
=2010=
===3SEP2010===
* Raj
** Status Casuarina Genome sequencing
** Outcrossing Needs to be compled and presendted on Monday
** Collect RNA samples for biomass gene targets
* Prasad
** Association DNA  completed and sent:
**  Working on 50 additional genes and organising for primer sysnthesis
** Planning on staructure analysis before the CSIRO
* Ashok
** Validation population (targetting Biomass genes): Completed the 580 plants DNA isolation
** Quality and Quantity check completed
** Making samples ready for Sending
* Nagabhushana
** SSR: Ordered 50 additional primers | Checking is in progress
** Hybrid confirmation: Needs to re-collect the samples
** TD-GD discussion
===Appraisal-APR2010===
:[[Appraisal]]
===4NOV2010===
* Raj
** Pollen dynamics - completed - MLTR - to be digged through..
** SSR Pipe - completed and pilot set sent for oligo synthesis
** Casuarina genome sequecing - status - Presentation completed. Next with Pradeep, Kulkarni
** TD-GD scoring, analysis and depiction
** Tilling Genes confirmed
** Transcriptomics-Tobacco: Mapping ESTs using '''bFast''' (new strategy)
** Digged thorough Gene prediction (completed genewise and genscan)
** Completed : 2010-11 budget, Flavour vision presentation
* Prasad
** Performance slides
** Finalyse the money part in CSIRO trip
** Could finalise 25 genes of growth related with partial sequences
** To lead stage wise expression studies of isolated genes
* Ashoke
** Performance slides
** Finalyse 2 selective profile this week in SEX marker
** His part of TD-GD over
* Bhushan
** Performance slides
** Sent pilot set of Ortho-SSR and follow up
** Remaining Gels of TD-GD (5 more)
 
=2009=
===15MAY2009===
* Discussion on Introduction
* Discussion on Introduction
* Nagabhusan
* Nagabhusan
Line 10: Line 57:
** Reviewed the work
** Reviewed the work
** Discussed on Expansin localization
** Discussed on Expansin localization
=22MAY2009=
===22MAY2009===
* Discussed on Poster
* Discussed on Poster
** Association mapping
** Association mapping
** Molecular markers as tools [ITC clones diversity; Hybrid confirmation; Model Natural Hybridization; Sex in Casuarina]
** Molecular markers as tools [ITC clones diversity; Hybrid confirmation; Model Natural Hybridization; Sex in Casuarina]
* XET gene (Ashoke) PCR amplification was trouble shooted (by Prasad)
* XET gene (Ashoke) PCR amplification was trouble shooted (by Prasad)
=29MAY2009=
===05JUN2009===
 
=05JUN2009=
* Progress
* Progress
** Raj
** Raj
Line 40: Line 85:
*** Gene walking
*** Gene walking
*** Expression studies
*** Expression studies
 
===12JUN2009===
=12JUN2009=
* Proof of principle - evagen - what followhup
* Proof of principle - evagen - what followhup
** It is possible to outsource the proof of principle
** It is possible to outsource the proof of principle
Line 55: Line 99:
** ASHOK : Expansin Walking Issues
** ASHOK : Expansin Walking Issues
** NAGA : Missing
** NAGA : Missing
 
===19JUN2009===
=19JUN2009=
* Status
* Status
** RAJ: Primer Screening ; Suggested to use the existing primers
** RAJ: Primer Screening ; Suggested to use the existing primers
Line 67: Line 110:
** Prasad
** Prasad
***
***
 
===26JUN2009===
=26JUN2009=
* Agenda
* Agenda
** Sample collection | Stress makes any difference ?
** Sample collection | Stress makes any difference ?
Line 75: Line 117:
** Raj
** Raj
*** Primers are fine, but HS Agarose is non-sense
*** Primers are fine, but HS Agarose is non-sense
 
===17AUG2009===
=17AUG2009=
*Common Stuff
*Common Stuff
** DNTPs (Requirement from my side 600ul of 80ug)
** DNTPs (Requirement from my side 600ul of 80ug)
Line 97: Line 138:
** Status on SSR
** Status on SSR
** Status on gene isolation
** Status on gene isolation
=4NOV2010=
* Raj
** Pollen dynamics - status to be finalised
** SSR Pipe - completed and pilot set sent for oligo synthesis
** Casuarina genome sequecng status
** TD-GD scoring, analysis and depiction
* Prasad
** Performance slides
** Finalyse the money part in CSIRO trip
** Could finalise 25 genes of growth related with partial sequences
** To lead stage wise expression studies of isolated genes
* Ashoke
** Performance slides
** Finalyse 2 selective profile this week in SEX marker
** His part of TD-GD over
* Bhushan
** Performance slides
** Sent pilot set of Ortho-SSR and follow up
** Remaining Gels of TD-GD (5 more)

Latest revision as of 08:41, 3 September 2010


2010

3SEP2010

  • Raj
    • Status Casuarina Genome sequencing
    • Outcrossing Needs to be compled and presendted on Monday
    • Collect RNA samples for biomass gene targets
  • Prasad
    • Association DNA completed and sent:
    • Working on 50 additional genes and organising for primer sysnthesis
    • Planning on staructure analysis before the CSIRO
  • Ashok
    • Validation population (targetting Biomass genes): Completed the 580 plants DNA isolation
    • Quality and Quantity check completed
    • Making samples ready for Sending
  • Nagabhushana
    • SSR: Ordered 50 additional primers | Checking is in progress
    • Hybrid confirmation: Needs to re-collect the samples
    • TD-GD discussion

Appraisal-APR2010

Appraisal

4NOV2010

  • Raj
    • Pollen dynamics - completed - MLTR - to be digged through..
    • SSR Pipe - completed and pilot set sent for oligo synthesis
    • Casuarina genome sequecing - status - Presentation completed. Next with Pradeep, Kulkarni
    • TD-GD scoring, analysis and depiction
    • Tilling Genes confirmed
    • Transcriptomics-Tobacco: Mapping ESTs using bFast (new strategy)
    • Digged thorough Gene prediction (completed genewise and genscan)
    • Completed : 2010-11 budget, Flavour vision presentation
  • Prasad
    • Performance slides
    • Finalyse the money part in CSIRO trip
    • Could finalise 25 genes of growth related with partial sequences
    • To lead stage wise expression studies of isolated genes
  • Ashoke
    • Performance slides
    • Finalyse 2 selective profile this week in SEX marker
    • His part of TD-GD over
  • Bhushan
    • Performance slides
    • Sent pilot set of Ortho-SSR and follow up
    • Remaining Gels of TD-GD (5 more)

2009

15MAY2009

  • Discussion on Introduction
  • Nagabhusan
    • Reviewed the work
    • Discussed on siRNA feasibilities
  • Prasad
    • Reviewed the work
    • Need the deliverables to be concised
  • Ashoke
    • Reviewed the work
    • Discussed on Expansin localization

22MAY2009

  • Discussed on Poster
    • Association mapping
    • Molecular markers as tools [ITC clones diversity; Hybrid confirmation; Model Natural Hybridization; Sex in Casuarina]
  • XET gene (Ashoke) PCR amplification was trouble shooted (by Prasad)

05JUN2009

  • Progress
    • Raj
      • Project GeneFlow
        • DNA Isolation from parents completed | Quantified | Working solution made
        • Plants resown for gap filling of projenies
        • Plants will be ready in 10 days time
        • Periodical thinning is suggested for DNA isolation from Monday
        • Primer screening to be started
      • Project :: Frankia
        • DNA isolated from 3 species | pathetic quantity
        • Primers are ready for PCR
    • Prasad
      • Gene walking
      • Expression studies
    • Ashoke
      • Gene Walking
        • Expansin Gene | Cloning completed | to be sent for sequencing
      • Expression studies
        • Ready Sample collection form different aged plants
    • Nagabhusana
      • Gene walking
      • Expression studies

12JUN2009

  • Proof of principle - evagen - what followhup
    • It is possible to outsource the proof of principle
  • RAC - by Mid JULY
  • Casuarina Plan
    • Accepted to get it done from Kovai
    • Species : C. juhuniana
    • Male Female bulks are possible
  • Change in timings to 3.30 pm on Friday
  • Status
    • RAJ : DNA Isolation
    • PRASAD : Close to 80% of the gene enrichment protocol
    • ASHOK : Expansin Walking Issues
    • NAGA : Missing

19JUN2009

  • Status
    • RAJ: Primer Screening ; Suggested to use the existing primers
    • Ashoke
      • Expansin - the primers didn't work. To design new primers (degenerate)
      • RACE - started and amplified first set of products
      • Plan for Seminar
    • Nagabhusana
      • CES A: Sent for sequencing
    • Prasad

26JUN2009

  • Agenda
    • Sample collection | Stress makes any difference ?
    • AFLP Kit ordering
    • Raj
      • Primers are fine, but HS Agarose is non-sense

17AUG2009

  • Common Stuff
    • DNTPs (Requirement from my side 600ul of 80ug)
    • Acrylamide plates order follow up - Nagabhushan
    • AFLP kit status - Ashoke to forward the message
    • LabNotebook, how do we deal it - sign every week
    • Equipments to Gurpreet: 1 PCR, 1 RT, 1 table top Centrifuge
    • Paper writing
    • SOL Meeting 2009 | last date for abstract 25AUG2009 - nothing this time
  • Raj
    • Outcrssing rate work looks intensive
    • Prasad already had a procedure for beat beating in DNA isoaltion
  • Prasad
    • Status - Gene enrichment protocol
    • Problem with trasformation - trouble shooting it
  • Ashoke
    • Status
    • Problem with trasformation - trouble shooting it
  • Bhushan
    • Status on SSR
    • Status on gene isolation
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