Tilling Genes: Difference between revisions
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* Identify SNP | * Identify SNP | ||
* Screen the mutants with specific strategy | * Screen the mutants with specific strategy | ||
=Conformation of Selected Genes= | =Conformation of Selected Genes= | ||
* Blast against methylated (TGI) sequences | * Blast against methylated (TGI) sequences | ||
** 7 selected genes | ** 7 selected genes | ||
<pre> | <pre> | ||
ANTL_AY461432 | |||
LEMTS1_Tomato)Linalool_Synthase_AY840091 | |||
LeMTS2 | |||
NtLs_EU935581_Lateral_suppressor | |||
AtTRIPTYCHON | |||
AtPPD2 | |||
AtPPD1 | |||
</pre> | |||
** Blastn against TGI [singlet and contig] | |||
** Results with TGI singlets | |||
<pre> | |||
Out of the 7 genes, | |||
ANTL_AY461432 | |||
NtLs_EU935581_Lateral_suppressor | |||
have better homology in Tobacco genome sequence, there fore pulling out | |||
the region in tobacco may not of big trouble | |||
The remaining genes, it is better to design primers on the mapped | |||
region of Tobacco to pull out the region in Tobacco | |||
</pre> | </pre> | ||
Latest revision as of 15:54, 9 January 2010
TILLING is to identify the mutants with trait of interest.
Strategy
- Develop a TILLING population
- Select/isolate the gene of interest (direct or orthologous)
- Identify SNP
- Screen the mutants with specific strategy
Conformation of Selected Genes
- Blast against methylated (TGI) sequences
- 7 selected genes
ANTL_AY461432 LEMTS1_Tomato)Linalool_Synthase_AY840091 LeMTS2 NtLs_EU935581_Lateral_suppressor AtTRIPTYCHON AtPPD2 AtPPD1
- Blastn against TGI [singlet and contig]
- Results with TGI singlets
Out of the 7 genes, ANTL_AY461432 NtLs_EU935581_Lateral_suppressor have better homology in Tobacco genome sequence, there fore pulling out the region in tobacco may not of big trouble The remaining genes, it is better to design primers on the mapped region of Tobacco to pull out the region in Tobacco