SNP Filtering Plans

Base Calling

Minimum Read depth
Based on Phred scores
1% error rate
Alignment (Trade off between accuracy and read depth)
Recalibration of Pherd scores
- Essential
- Phred score of Q should be = 10 to the power Q by 10 or less. This is done by alignning with the reference with the known SNPs
Homo and Heterozgous SNPs in a diploid
- Homozygous -> If an SNP (different than ref) base is counted across the read depth to be more than 80%
- Hetorozygous -> If an SNP (different than ref) base is counted across the read depth to be less than 80%
- Sequence/alignment Error -> If an SNP based is counted to be less than 10%
- This is true of the depth is minimum of 20x
Accuracy in SNP calling
- Accuracy can be improved from single(Ref vs one sample) to multi samples (Ref vs several samles)
- Possible accuracy by read depth based SNP calling is 85%
- Possible accuracy by LD (linkage disequilibrium) is >95%