Comparitive Genomes

Eukaryotes

1. History
   1. Initial approach were based on crm size > karyotype > banding > painting (G-banding was most common)
   2. Structure of Typical Eukaryotic gene
2. Working with Eukaryotic genome
   1. Physical and genetic mapping
   2. Sequencing: Holy grail of comparative genomics
      1. Saccharomyces -> first eukaryotic genome sequenced
      2. HGP -. completed in 2001. Still number of genes is questionable. (gene predicting programmes fail to find ORF; pseudogenes, alternate splicing)
      3. Within pop seq variation makes the comparative genomics difficult
      4. Fugu -> second sequenced