Pollen dynamics

Fruiting Status - Mulugu - Dec2008

• Resources:

Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers

Tree Genetics & Genomes (2008) 4:37–47

• Vishwanath
  • Title: Pollen flow in Eucalyptus seed orchards as determined by paternity analysis

---

• Work plan: Pollen_Dynamics
• Logistics
  • 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
  • DNA Isolation: 375 samples
  • PCR reaction : 375 * 20 = 7500
  • Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)

---

• Requirements
  • Trays
  • Qiagen DNA isolation kit: 2 (each can handle 250 samples)
  • Ampli Taq 2 vials
  • High resolution Agarose: 100g

---

• Strategy
  • Seeds sowing
  • Leaf sample collection from parental trees (15) and seedlings after one and half month
  • DNA isolation from 375 samples
  • Take representative sample and screen the primers to select 20 good
  • PCR with 375 x 20 SSR primers
  • Score them and make an automated DB for analysis
  • Make meaningful predictions for the Mulugu SSO

---