Pollen dynamics

Fruiting Status - Mulugu - Dec2008

• Resources:

Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers

Tree Genetics & Genomes (2008) 4:37–47

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• Work plan: Pollen_Dynamics
• Logistics
  • 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
  • DNA Isolation: 375 samples
  • PCR reaction : 375 * 20 = 7500
  • Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)

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• Requirements
  • Trays
  • Qiagen DNA isolation kit: 2 (each can handle 250 samples)
  • Ampli Taq 2 vials
  • High resolution Agarose: 100g

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