Pollen Dynamics

2008 Fall

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Pollen and Dynamics and outcrossing rate of CSO and SSO
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Study CSO (Mulugu), SSO (ongole)
	
Questions that we ask:	
		
	1. What is outcrossing rate of best performing clones/Seed origin trees?
	2. Is there a seasonal variation in the outcrossing rate?
	3. How male contribution affects the fertility?
	4. How physical distance affects the fertility?
	5. Does fertility patters changes with seasons?
	6. Does SSO is economically viable than CSO?

Materials
	* CSO (Mulugu), SSO (ongole)
Methods
	* Positional Mapping of population
		* Layout of all the tress in CSO/SSO
		* Developing a scaled map of trees in CSO/SSO
		
		* Fertility estimation
			* Flower/fruit count of all the trees in CSO/SSO in 2 seasons
		
	* Tree selection (5)
		* Based on fertility
		* Seed collection
		* Selection of 10 Parternal trees

	* Outcrossing rate
		* Season 1 (Sep - Jan):
			* DNA from 135 progenies (5*25 - maternal) + 10 (paternal)
			* SSR markers (20)
			* Season 2 (Apr - Aug)
				* Same as above
			
			Peternity analysis (CERVUS, etc)
		
	* Analysis
		* Estimation of fertility in peternal and maternal trees.
		* Relation | Fertility Vs physical distance
		* Relation | Fertility Vs outcrossing
		
		* Interpretation

	* Publication(s):
		season 1:
			Answer for the questions 1, 3, 4, ~5
		Season 2:
			Answer for questions 1 to 6