Casuarina SSR: Difference between revisions
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=Scientific Assumptions= | =Scientific Assumptions= | ||
EST SSRs are supposedly conserved across species therefore higher | EST SSRs are supposedly conserved across species therefore higher species transferability is expected | ||
=Strategy= | =Strategy= | ||
Revision as of 03:41, 29 December 2011
New SSRs Pipeline Target
- Designing Cross species EST SSRs from Casuarina
- Author: Ashok Kulkarni and Rajkumar
- Timeline
- Designing Primers: DEC 2011
- Oligo synthesis : JAN 2012
- Standardisation and Validation : MAR 2010
- Manuscript : JUN 2010
Scientific Assumptions
EST SSRs are supposedly conserved across species therefore higher species transferability is expected
Strategy
- C. glauca ESTs
- Develop unigenes
- Generate statistics
- Filter the sequence
- Mine for repeat motifs
- Design Primers