Casuarina SSR: Difference between revisions
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=Strategy= | =Strategy= | ||
* C. glauca ESTs | |||
* Develop unigenes | |||
* Generate statistics | |||
* Filter the sequence | |||
* Mine for repeat motifs | |||
* Design Primers | |||
=Implementation= | =Implementation= | ||
Revision as of 03:38, 29 December 2011
New SSRs Pipeline Target
- Designing Cross species EST SSRs from Casuarina
- Author: Ashok Kulkarni and Rajkumar
- Timeline
- Designing Primers: DEC 2011
- Oligo synthesis : JAN 2012
- Standardisation and Validation : MAR 2010
- Manuscript : JUN 2010
Scientific Assumptions
EST SSRs are supposedly conserved across species therefore higher percentage of success in species transferability
Strategy
- C. glauca ESTs
- Develop unigenes
- Generate statistics
- Filter the sequence
- Mine for repeat motifs
- Design Primers