Transcriptomics-Tobacco: Difference between revisions

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** Sequencing of ESTs: SEP 2009
** Sequencing of ESTs: SEP 2009
** Assemble / pre annotation: NOV 2009
** Assemble / pre annotation: NOV 2009
** Print Array  : JAN 2010
** Printing Array  : JAN 2010
** Combining the protein data: MAR 2010
** Combining the protein data: MAR 2010
** Re annotation of hybridised '''genes'''  : APR 2010
** Re annotation of hybridised '''genes'''  : APR 2010

Revision as of 10:29, 4 January 2010

Tobacco Pipeline Target

  • Get the differentially expressed Genes in tobacco leaf
  • Author: Guru and Rajkumar and et al
  • Timeline
    • Sequencing of ESTs: SEP 2009
    • Assemble / pre annotation: NOV 2009
    • Printing Array  : JAN 2010
    • Combining the protein data: MAR 2010
    • Re annotation of hybridised genes  : APR 2010
    • Consenses of the 2 data for the selected genes (MAY 2010)
    • Validate selected genes (100) by RT: JUN 2010
    • Manuscript (after approval): JUL 2010
    • Lab validation of selected genes: DEC 2010
    • Get the final list of genes characterised : JAN 2011
    • Manuscript MAR 2011

Scientific Assumptions

Strategy

Implementation

  1. Some Tools
    1. gth_run
    2. trim_seq | Filter "N", size
    3. multifasta-fastas
    4. Mapping ESTs on tgi
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