Pollen dynamics: Difference between revisions
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** PCR reaction : 375 * 20 = 7500 | ** PCR reaction : 375 * 20 = 7500 | ||
** Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples) | ** Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples) | ||
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* Requirements | |||
** Trays | |||
** Qiagen DNA isolation kit: 2 (each can handle 250 samples) | |||
** Ampli Taq 2 vials | |||
** High resolution Agarose: 100g | |||
Revision as of 08:51, 25 March 2009
- Resources:
- Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers
- Tree Genetics & Genomes (2008) 4:37–47
- Work plan: Pollen_Dynamics
- Logistics
- 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
- DNA Isolation: 375 samples
- PCR reaction : 375 * 20 = 7500
- Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)
- Requirements
- Trays
- Qiagen DNA isolation kit: 2 (each can handle 250 samples)
- Ampli Taq 2 vials
- High resolution Agarose: 100g