Pollen dynamics: Difference between revisions
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* Work plan: [[Pollen_Dynamics]] | * Work plan: [[Pollen_Dynamics]] | ||
* Logistics | |||
** 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed | |||
** DNA Isolation: 375 samples | |||
** PCR reaction : 375 * 20 = 7500 | |||
** Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples) | |||
Revision as of 08:47, 25 March 2009
- Resources:
- Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers
- Tree Genetics & Genomes (2008) 4:37–47
- Work plan: Pollen_Dynamics
- Logistics
- 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
- DNA Isolation: 375 samples
- PCR reaction : 375 * 20 = 7500
- Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)