Casuarina SSR: Difference between revisions
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| Line 24: | Line 24: | ||
=Implementation= | =Implementation= | ||
===C. glauca ESTs=== | ===C. glauca ESTs=== | ||
* Total Number of ESTs: 34897 | * Total Number of ESTs: '''34897''' | ||
* | * Total number of C. glauca ESTs: '''34805''' | ||
===Develop unigenes=== | ===Develop unigenes=== | ||
Revision as of 03:45, 29 December 2011
New SSRs Pipeline Target
- Designing Cross species EST SSRs from Casuarina
- Author: Ashok Kulkarni and Rajkumar
- Timeline
- Designing Primers: DEC 2011
- Oligo synthesis : JAN 2012
- Standardisation and Validation : MAR 2010
- Manuscript : JUN 2010
Scientific Assumptions
EST SSRs are supposedly conserved across species therefore higher species transferability is expected
Strategy
- C. glauca ESTs
- Develop unigenes
- Generate statistics
- Filter the sequence
- Mine for repeat motifs
- Design Primers
Implementation
C. glauca ESTs
- Total Number of ESTs: 34897
- Total number of C. glauca ESTs: 34805