Revision as of 20:00, 3 January 2010

Target

title
- Pollen flow in Eucalyptus camaldulensis determined by paternity analysis using microsatellite markers
- Title: Pollen flow in Eucalyptus seed orchards as determined by paternity analysis
People
- Raj, Vishwanath, Kamal, Mohan

Logistics
- 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
- DNA Isolation: 375 samples
- PCR reaction : 375 * 20 = 7500
- Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)
Requirements
- Trays
- Qiagen DNA isolation kit: 2 (each can handle 250 samples)
- Ampli Taq 2 vials
- High resolution Agarose: 100g

The following 3 pages are in this category, out of 3 total.

@@ Line 8: / Line 8: @@
 =Strategy=
+[[Image:Fruiting.png|thumb|200px|right| Fruiting Status - Mulugu - Dec2008]]
 [[Pollen_Dynamics|Earlier Plan]]
-[[Image:Fruiting.png|thumb|200px|right| Fruiting Status - Mulugu - Dec2008]]
+** Seeds sowing
+** Leaf sample collection from parental trees (15) and seedlings after one and half month
+** DNA isolation from 375 samples
+** Take representative sample and screen the primers to select 20 good
+** PCR with 375 x 20 SSR primers
+** Score them and make an automated DB for analysis
+** Make meaningful predictions for the Mulugu SSO
 =Implementation=
+=== Work plan ===
+* Logistics
+** 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
+** DNA Isolation: 375 samples
+** PCR reaction : 375 * 20 = 7500
+** Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)
+* Requirements
+** Trays
+** Qiagen DNA isolation kit: 2 (each can handle 250 samples)
+** Ampli Taq 2 vials
+** High resolution Agarose: 100g