Pollen dynamics: Difference between revisions

Latest revision as of 17:24, 14 February 2017

Tree Genetics & Genomes (2008) 4:37–47

Vishwanath
- Title: Pollen flow in Eucalyptus seed orchards as determined by paternity analysis
Gene_Flow_Progress

Work plan: Pollen_Dynamics
Logistics
- 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
- DNA Isolation: 375 samples
- PCR reaction : 375 * 20 = 7500
- Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)

Requirements
- Trays
- Qiagen DNA isolation kit: 2 (each can handle 250 samples)
- Ampli Taq 2 vials
- High resolution Agarose: 100g

@@ Line 1: / Line 1: @@
+[[Image:Fruiting.png|thumb|200px|right| Fruiting Status - Mulugu - Dec2008]]
-[[Category:Projects]]
+* Resources:
-[[Category:Pollen_dynamics]]
+:[http://www.download.rajkumar.in/itc/readings/pollen/Pollen_flow_grandis_peternity.pdf Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers]
+: Tree Genetics & Genomes (2008) 4:37–47
+* [[Vishwanath]]
+** Title: Pollen flow in Eucalyptus seed orchards as determined by paternity analysis
+* [[Gene_Flow_Progress]]
+----
+* Work plan: [[Pollen_Dynamics]]
+* Logistics
+** 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
+** DNA Isolation: 375 samples
+** PCR reaction : 375 * 20 = 7500
+** Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)
+----
+* Requirements
+** Trays
+** Qiagen DNA isolation kit: 2 (each can handle 250 samples)
+** Ampli Taq 2 vials
+** High resolution Agarose: 100g
+----
+* Strategy
+** Seeds sowing
+** Leaf sample collection from parental trees (15) and seedlings after one and half month
+** DNA isolation from 375 samples
+** Take representative sample and screen the primers to select 20 good
+** PCR with 375 x 20 SSR primers
+** Score them and make an automated DB for analysis
+** Make meaningful predictions for the Mulugu SSO
+----
+[[Category:Readings]] [[Category:Rajkumar]] [[Category:Silvi]]