Pollen dynamics: Difference between revisions
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[http://www.download.rajkumar.in/itc/readings/pollen/Pollen_flow_grandis_peternity.pdf Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers] | [[Image:Fruiting.png|thumb|200px|right| Fruiting Status - Mulugu - Dec2008]] | ||
* Resources: | |||
:[http://www.download.rajkumar.in/itc/readings/pollen/Pollen_flow_grandis_peternity.pdf Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers] | |||
: Tree Genetics & Genomes (2008) 4:37–47 | |||
* [[Vishwanath]] | |||
** Title: Pollen flow in Eucalyptus seed orchards as determined by paternity analysis | |||
[[Category: | * [[Gene_Flow_Progress]] | ||
[[Category: | ---- | ||
[[Category: | * Work plan: [[Pollen_Dynamics]] | ||
* Logistics | |||
** 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed | |||
** DNA Isolation: 375 samples | |||
** PCR reaction : 375 * 20 = 7500 | |||
** Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples) | |||
---- | |||
* Requirements | |||
** Trays | |||
** Qiagen DNA isolation kit: 2 (each can handle 250 samples) | |||
** Ampli Taq 2 vials | |||
** High resolution Agarose: 100g | |||
---- | |||
* Strategy | |||
** Seeds sowing | |||
** Leaf sample collection from parental trees (15) and seedlings after one and half month | |||
** DNA isolation from 375 samples | |||
** Take representative sample and screen the primers to select 20 good | |||
** PCR with 375 x 20 SSR primers | |||
** Score them and make an automated DB for analysis | |||
** Make meaningful predictions for the Mulugu SSO | |||
---- | |||
[[Category:Readings]] [[Category:Rajkumar]] [[Category:Silvi]] | |||
Latest revision as of 17:24, 14 February 2017
- Resources:
- Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers
- Tree Genetics & Genomes (2008) 4:37–47
- Vishwanath
- Title: Pollen flow in Eucalyptus seed orchards as determined by paternity analysis
- Gene_Flow_Progress
- Work plan: Pollen_Dynamics
- Logistics
- 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
- DNA Isolation: 375 samples
- PCR reaction : 375 * 20 = 7500
- Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)
- Requirements
- Trays
- Qiagen DNA isolation kit: 2 (each can handle 250 samples)
- Ampli Taq 2 vials
- High resolution Agarose: 100g
- Strategy
- Seeds sowing
- Leaf sample collection from parental trees (15) and seedlings after one and half month
- DNA isolation from 375 samples
- Take representative sample and screen the primers to select 20 good
- PCR with 375 x 20 SSR primers
- Score them and make an automated DB for analysis
- Make meaningful predictions for the Mulugu SSO