Category:Biology: Difference between revisions
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=TD-GD in Euca= | =TD-GD in Euca= | ||
* Pilot experiment (for CMC) | * Pilot experiment (for CMC) | [[TD-GD_Pilot]] | ||
** Data point 1 (ITC clones existing PY/WY/Lignin vs ITC clones He) | ** Data point 1 (ITC clones existing PY/WY/Lignin vs ITC clones He) | ||
** Data point 2 (New clones PY/WY/Lignin vs its He) | ** Data point 2 (New clones PY/WY/Lignin vs its He) | ||
| Line 25: | Line 25: | ||
*** Normalise to find out the pattern | *** Normalise to find out the pattern | ||
*** Interpret for each type of data | *** Interpret for each type of data | ||
<pre> | |||
=============== | |||
GD-TD in Euca | |||
=============== | |||
What it is? | |||
It is a graph that plots genetic diversity and trait diversity in consensus units, which depicts the status, essentially helps in decision making on crop improvement programe such as | |||
* To ascertain the worthiness of the strategies amalgamated to improve the trait diversity | |||
* To provide the scope for necessitating the improvement for genetic diversity | |||
Objective: | |||
To establish the GD-TD status of Indian eucalyptus core population | |||
Strategy | |||
* Representative materials (10) from each pool for diversity estimates (analysis together) | |||
* Existing realistic data (Mor. Traits) | |||
* Expected heterozygosity (He) vs each mor. traits | |||
* Estimate the He-Ho to acertain the quantum if Hardy wainberg forces on each population | |||
* plot the data | |||
* Interpret to provide breeding strategy for each population | |||
* Publication (Timeline DEC 2010) | |||
Real scale Materials | |||
SNP Material (2 provinace from Australia) | |||
IFGTP (Collection) | |||
ITC clones | |||
Hybrids of Ec vs Ep | |||
New Species pool | |||
E. urophylla collection (South Africa) | |||
New clones selection | |||
==================================================================== | |||
Pilot experiment | |||
Plot GD-TD of New clones selection for 'PY' vs 'He' for 10 selected new clones | |||
Strategy | |||
* 10 New clones + 5 ITC clones were selected for analysis | |||
* 'He' 'Ho' were estimated using 15 selected SSR markers | |||
* 'PY' was estimated using NIR | |||
* Analysis | |||
* Heterozygosity was estimated for individual groups (ITC and New clones) as well as combined ITC+New clones | |||
* For the indidual He values of new clones, each new clone data was combined with all ITC clones | |||
* Values were ploted as below | |||
* 'He' of indidual New cloens vs pulp yield | |||
* Mean 'He' vs mean pulp yiled of new clones | |||
* Mean 'He' vs mean pulp yiled of ITC clones | |||
* Mean 'He' vs mean pulp yiled of ITC+new clones | |||
* Results and 'take home' from pilot experiment with new clone | |||
* "He" is ~0.69 for ITC and NC, when analyzed individually. Though ITC clones and new clones "little" differ mathematically, they do not differ significantly, both group have good allelic diversity, that tells us the potential for improvement. | |||
* When data is analyzed as a whole (ITC+NC), the "He" deviates (0.72) then either of the individual group. This essentially means that there are different set of alleles (so its frequency) present in each group. Therefore NC and ITC types deserve the definition of "population" that arise from different pedigree. | |||
* In all the cases, Ho is ~0.4 and the deviation of He and Ho ("He-Ho") is really notable(~0.3). This tells us that both ITC and NC group have undergone considerable amount of "disturbing" forces (as defined Hardy weinberg law) | |||
Bussiness interest: | |||
* There is a shift from one | |||
</pre> | |||
Latest revision as of 04:26, 21 November 2016
TD-GD
- What it is? It is a graph that plots genetic diversity and trait diversity in consensus units, which depicts the status, essentially helps in decision making on crop improvement programe such as
- To ascertain the worthiness of the strategies amalgamated to improve the trait diversity
- To provide the scope for necessitating the improvement for genetic diversity
TD-GD in Euca
- Pilot experiment (for CMC) | TD-GD_Pilot
- Data point 1 (ITC clones existing PY/WY/Lignin vs ITC clones He)
- Data point 2 (New clones PY/WY/Lignin vs its He)
- Time line JAN 2010
- Real scale [for publication]
- Timeline DEC 2010
- Materials
- Representative SNP Trial
- IFGTP (F2 seggregants)
- ITC clones
- New clones
- Hybrids of Ec vs Ep
- New Species pool
- E. urophylla collection
- Strategy
- Representative materials (10) from each pool for diversity estimates (analysis together)
- Existing realistic data (Mor. Traits)
- Plot He vs each mor. traits
- Normalise to find out the pattern
- Interpret for each type of data
===============
GD-TD in Euca
===============
What it is?
It is a graph that plots genetic diversity and trait diversity in consensus units, which depicts the status, essentially helps in decision making on crop improvement programe such as
* To ascertain the worthiness of the strategies amalgamated to improve the trait diversity
* To provide the scope for necessitating the improvement for genetic diversity
Objective:
To establish the GD-TD status of Indian eucalyptus core population
Strategy
* Representative materials (10) from each pool for diversity estimates (analysis together)
* Existing realistic data (Mor. Traits)
* Expected heterozygosity (He) vs each mor. traits
* Estimate the He-Ho to acertain the quantum if Hardy wainberg forces on each population
* plot the data
* Interpret to provide breeding strategy for each population
* Publication (Timeline DEC 2010)
Real scale Materials
SNP Material (2 provinace from Australia)
IFGTP (Collection)
ITC clones
Hybrids of Ec vs Ep
New Species pool
E. urophylla collection (South Africa)
New clones selection
====================================================================
Pilot experiment
Plot GD-TD of New clones selection for 'PY' vs 'He' for 10 selected new clones
Strategy
* 10 New clones + 5 ITC clones were selected for analysis
* 'He' 'Ho' were estimated using 15 selected SSR markers
* 'PY' was estimated using NIR
* Analysis
* Heterozygosity was estimated for individual groups (ITC and New clones) as well as combined ITC+New clones
* For the indidual He values of new clones, each new clone data was combined with all ITC clones
* Values were ploted as below
* 'He' of indidual New cloens vs pulp yield
* Mean 'He' vs mean pulp yiled of new clones
* Mean 'He' vs mean pulp yiled of ITC clones
* Mean 'He' vs mean pulp yiled of ITC+new clones
* Results and 'take home' from pilot experiment with new clone
* "He" is ~0.69 for ITC and NC, when analyzed individually. Though ITC clones and new clones "little" differ mathematically, they do not differ significantly, both group have good allelic diversity, that tells us the potential for improvement.
* When data is analyzed as a whole (ITC+NC), the "He" deviates (0.72) then either of the individual group. This essentially means that there are different set of alleles (so its frequency) present in each group. Therefore NC and ITC types deserve the definition of "population" that arise from different pedigree.
* In all the cases, Ho is ~0.4 and the deviation of He and Ho ("He-Ho") is really notable(~0.3). This tells us that both ITC and NC group have undergone considerable amount of "disturbing" forces (as defined Hardy weinberg law)
Bussiness interest:
* There is a shift from one
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