Latest revision as of 04:24, 21 November 2016

Target

Outcrossing rate is partly a "G" factor and can be used as a selection index in seed orchard to maximise the reproductive success

Earlier Plan
Seeds sowing
Leaf sample collection from parental trees (15) and seedlings after one and half month
DNA isolation from 375 samples
Take representative sample and screen the primers to select 20 good
PCR with 375 x 20 SSR primers
Score them and make an automated DB for analysis
Make meaningful predictions for the Mulugu SSO

Logistics
- 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
- DNA Isolation: 375 samples
- PCR reaction : 375 * 20 = 7500
- Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)
Requirements
- Trays
- Qiagen DNA isolation kit: 2 (each can handle 250 samples)
- Ampli Taq 2 vials
- High resolution Agarose: 100g

The following 3 pages are in this category, out of 3 total.

@@ Line 1: / Line 1: @@
-[[Image:outcrossing.png|thumb|200px|right]]
 = Target=
 * Title Pollen flow in Eucalyptus camaldulensis determined by paternity analysis
 * People: Raj, Vishwanath, Kamal, Mohan
 =Scientific Assumptions=
-Outcrossing rate is partly a "G" factor and can be used as a selection index in breeding population in seed orchards
+Outcrossing rate is partly a "G" factor and can be used as a selection index in seed orchard to maximise the reproductive success
+[[Image:outcrossing.png|thumb|250px|right]]
 =Strategy=
 * [[Pollen_Dynamics|Earlier Plan]]