Category:Pollen: Difference between revisions
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= Target= | = Target= | ||
* | * Title Pollen flow in Eucalyptus camaldulensis determined by paternity analysis | ||
* People: Raj, Vishwanath, Kamal, Mohan | |||
* | |||
=Scientific Assumptions= | =Scientific Assumptions= | ||
Outcrossing rate is partly a "G" factor and can be used as a selection index in seed orchard to maximise the reproductive success | |||
[[Image:outcrossing.png|thumb|250px|right]] | |||
=Strategy= | |||
* [[Pollen_Dynamics|Earlier Plan]] | |||
* Seeds sowing | |||
* Leaf sample collection from parental trees (15) and seedlings after one and half month | |||
* DNA isolation from 375 samples | |||
* Take representative sample and screen the primers to select 20 good | |||
* PCR with 375 x 20 SSR primers | |||
* Score them and make an automated DB for analysis | |||
* Make meaningful predictions for the Mulugu SSO | |||
=Implementation= | =Implementation= | ||
=== Work plan === | |||
* Logistics | |||
** 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed | |||
** DNA Isolation: 375 samples | |||
** PCR reaction : 375 * 20 = 7500 | |||
** Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples) | |||
* Requirements | |||
** Trays | |||
** Qiagen DNA isolation kit: 2 (each can handle 250 samples) | |||
** Ampli Taq 2 vials | |||
** High resolution Agarose: 100g | |||
Latest revision as of 04:24, 21 November 2016
Target
- Title Pollen flow in Eucalyptus camaldulensis determined by paternity analysis
- People: Raj, Vishwanath, Kamal, Mohan
Scientific Assumptions
Outcrossing rate is partly a "G" factor and can be used as a selection index in seed orchard to maximise the reproductive success
Strategy
- Earlier Plan
- Seeds sowing
- Leaf sample collection from parental trees (15) and seedlings after one and half month
- DNA isolation from 375 samples
- Take representative sample and screen the primers to select 20 good
- PCR with 375 x 20 SSR primers
- Score them and make an automated DB for analysis
- Make meaningful predictions for the Mulugu SSO
Implementation
Work plan
- Logistics
- 15 Maternal/Paternal* 25 progenies = 375 plants to be analyzed
- DNA Isolation: 375 samples
- PCR reaction : 375 * 20 = 7500
- Gel Run: 7500/60 = 125 runs in acrylamide (30+30 samples)
- Requirements
- Trays
- Qiagen DNA isolation kit: 2 (each can handle 250 samples)
- Ampli Taq 2 vials
- High resolution Agarose: 100g
Pages in category "Pollen"
The following 3 pages are in this category, out of 3 total.