https://www.rajkumar.in/index.php?action=history&feed=atom&title=MicroRNA_Evolution 2026-07-09T19:18:34Z Revision history for this page on the wiki MediaWiki 1.46.0 https://www.rajkumar.in/index.php?title=MicroRNA_Evolution&diff=1805&oldid=prev 2010-06-17T11:14:54Z

<p>moved <a href="/index.php/MicroRNA" title="MicroRNA">MicroRNA</a> to <a href="/index.php/MicroRNA_Evolution" title="MicroRNA Evolution">MicroRNA Evolution</a></p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <tr class="diff-title" lang="en"> <td colspan="1" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="1" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:14, 17 June 2010</td> </tr><tr><td colspan="2" class="diff-notice" lang="en"><div class="mw-diff-empty">(No difference)</div> </td></tr></table>

Raj https://www.rajkumar.in/index.php?title=MicroRNA_Evolution&diff=124&oldid=prev 2008-08-23T03:08:48Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 03:08, 23 August 2008</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l51">Line 51:</td> <td colspan="2" class="diff-lineno">Line 51:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*[http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-313X.2006.02697.x Conservation and divergence of plant microRNA genes Plant J. 2006 Apr;46(2):243-59]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*[http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-313X.2006.02697.x Conservation and divergence of plant microRNA genes Plant J. 2006 Apr;46(2):243-59]</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [[Media:Mirna_protocols.pdf | mirna protocols]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [[Media:Mirna_protocols.pdf | mirna protocols]]</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Projects]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Bioinformatics]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:MicroRNA]]</ins></div></td></tr> </table>

Raj https://www.rajkumar.in/index.php?title=MicroRNA_Evolution&diff=100&oldid=prev 2008-08-23T02:53:16Z

<p>New page: ==Tittle== * Conservation of MicroRNA genes across genomes ==Objective== # To study the miRNA similarity between species # miRNA as a classifier between species. # Defining common motifs/...</p> <p><b>New page</b></p><div>==Tittle==<br /> * Conservation of MicroRNA genes across genomes<br /> <br /> ==Objective==<br /> # To study the miRNA similarity between species<br /> # miRNA as a classifier between species.<br /> # Defining common motifs/signature in miRNAs of human (Primary, Mature and Precursor)<br /> # A phylogenetic tree for miRNAs of Human, mouse and other species.<br /> # Possible subclassification of miRNAs.<br /> <br /> ==Data==<br /> Kept in mustard /home/rajkumar/mirna/<br /> <br /> * mature.fa - 4434 entries - 21 - 23 bases<br /> * mirna precursor.txt - 4584 entries - ~ 60 - 130 bases<br /> <br /> ==Programme==<br /> === &#039;&#039;&#039;Search for mirna in Mimulus&#039;&#039;&#039; (from Mimulus unigenes)===<br /> # Driving question<br /> ## Does miRNA really has phylogenetic signal across Asterids?<br /> ## If so, how far the miRNA is conserved/diverged?<br /> ## Conservation/divergence of Mimulus miRNAs<br /> # Data<br /> ## Unigenes from Arabidopsis, <br /> ## Tomato<br /> ## Rice<br /> ## Mimulus<br /> ## Populus<br /> # Plan of work<br /> ## Assemble ESTs and proteins of available systems<br /> ## Blast against protein database<br /> ## Remove the protein coding ESTS<br /> ## Again blast against EST database (0-4 mismatches)<br /> ## Predict the RNA structure by mfod 3.1<br /> ## Harpin Candidates<br /> ## Examine the annotations<br /> ## Noval potential miRNa genes<br /> ## Align the miRNA genes<br /> ## Perform phylogenetic analysis<br /> ===&#039;&#039;&#039;Conservation of MicroRNA genes across genomes&#039;&#039;&#039;===<br /> # Assemble the mature.fa with Plant and animal miRNA seq sorted out separately <br /> # Align the mature.fa sequences using Genome Threader or clustal family software<br /> # Find out the coordinates of most common motifs (might need to play with the stringency parameters a bit in alignment)<br /> # Might need a subclassifications, to visualize in Apollo<br /> <br /> ==Analysis==<br /> <br /> ==Resutlts==<br /> <br /> ==Related Articles==<br /> *[http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-313X.2006.02697.x Conservation and divergence of plant microRNA genes Plant J. 2006 Apr;46(2):243-59]<br /> * [[Media:Mirna_protocols.pdf | mirna protocols]]</div>

Raj